Journal: Journal of Neuroinflammation

Article Title: CXCL7/CXCR2 in the paraventricular thalamus mediates obesity-related pain

doi: 10.1186/s12974-025-03679-x

Figure Lengend Snippet: Increased inflammatory and oxidative stress levels in the PVT of DIO mice. A Quantification of CXCL7 in plasma from control or DIO mice. n = 3 mice per group, P = 0.0003. B and C Representative images ( B ) and summarized data ( C ) of the expression of CXCL7 in PVT Glu neurons from control and DIO mice. n = 5 mice per group, with one slice from each mouse. P < 0.0001. Scale bars, 10 μm. D - H Representative images ( D ) and quantification of IBA1 + cell numbers ( E ), intensity ( F ) and Imaris-based semiautomatic quantification of cell morphometry, including total branch length ( G ) and points ( H ) of IBA1 + -microglia from control and DIO mice. n = 5 mice per group for quantification of cell number and intensity, with one slice from each mouse. P (number) = 0.0081, P (intensity) = 0.0122. n= 6 mice for the control group and n = 7 mice for the DIO group in the quantification of branch length and points, with one slice from each mouse. P (length) = 0.014, P (intensity) = 0.0001. Scale bars, 10 μm (overview) and 3 μm (inset and rendering). I and J Representative images ( I ) and summarized data ( J ) of GPX4 in PVT Glu neurons from control and DIO mice. n = 6 mice per group, with one slice from each mouse. P = 0.0012. Scale bars, 10 μm. Statistical analysis was performed using Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Seven days later, a volume of 50 ng/μl CXCL7 protein (HY- P75698 , MCE, USA) dissolved in artificial cerebrospinal fluid (ACSF) was infused into the lateral ventricles (1 μl), or PVT (300 nl) was given once a day for three days in a row.

Techniques: Clinical Proteomics, Control, Expressing

Journal: Journal of Neuroinflammation

Article Title: CXCL7/CXCR2 in the paraventricular thalamus mediates obesity-related pain

doi: 10.1186/s12974-025-03679-x

Figure Lengend Snippet: Infusion of CXCL7 improved PVT Glu neuronal activity and induced pain hypersensitivity in control mice. A Schematic of the experimental procedure. B Image showing the site of the cannula implanted in the lateral ventricle of control mice. Scale bar, 500 μm. C and D Effects of infusion of CXCL7 in the lateral ventricle on the pain threshold in control mice treated with ACSF or CXCL7, using von Frey test ( C ) and Hargreaves tests ( D ). n = 5 mice per group, P = 0.0079 ( C ), P = 0.0007 ( D ). E Representative heatmaps of travel trajectory of control mice treated with ACSF or CXCL7 in the CPA test. F and G Summarized data of aversion index ( F ) and preference index ( G ) for ( E ), n = 7 mice per group. P < 0.0001 ( F and G ). ( H ) Image showing the site of the cannula implanted in the PVT of control mice. Scale bar, 500 μm. I and J Representative images ( I ) and summarized data ( J ) of CXCL7 on PVT Glu neurons in control mice treated with ACSF or CXCL7. n = 6 mice, with one slice from each mouse, P < 0.0001. Scale bar, 10 μm. K and L Representative images ( K ) and summarized data ( L ) of c-Fos in PVT from control mice treated with ACSF or CXCL7. n = 5 mice, with one slice from each mouse. P = 0.0061. Scale bar, 200 μm. M and N Sample traces ( M ) and summarized number of spikes ( N ) in PVT Glu neurons recorded from control mice treated with ACSF or CXCL7. n = 14 cells from 4 mice per group. P (time × column factor) = 0.0019, P (time) < 0.0001, P (column factor) = 0.001, F (1, 26) = 13.79. O and P Effects of the infusion of CXCL7 in PVT Glu neurons on the pain threshold using the von Frey test ( O ) and Hargreaves tests ( P ) of control mice treated with ACSF or CXCL7. n = 7 mice per group, P = 0.0002 ( O ), P = 0.0015 ( P ). Q Representative heatmaps of travel trajectory of control mice treated with ACSF or CXCL7 within PVT in the CPA test. ( R and S ) Summarized data of aversion index ( R ) and preference index ( S ) for ( Q ), n = 7 mice per group. P = 0.0003 ( R and S ). Statistical analysis was performed using Student’s t test in ( C ), ( D) , ( F) , ( G) , ( J) , ( L) , ( O) , ( P) , ( R) and ( S ), and two-way ANOVA followed by Bonferroni’s post hoc correction in ( N ). ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Seven days later, a volume of 50 ng/μl CXCL7 protein (HY- P75698 , MCE, USA) dissolved in artificial cerebrospinal fluid (ACSF) was infused into the lateral ventricles (1 μl), or PVT (300 nl) was given once a day for three days in a row.

Techniques: Activity Assay, Control

Journal: Journal of Neuroinflammation

Article Title: CXCL7/CXCR2 in the paraventricular thalamus mediates obesity-related pain

doi: 10.1186/s12974-025-03679-x

Figure Lengend Snippet: Mechanism of CXCL7-CXCR2 interaction in PVT Glu →BLA Glu neuronal circuit promotes the onset of obesity-related pain. In obese mice, elevated expression of the chemokine, CXCL7, results in the binding of CXCR2 receptors of PVT Glu neurons, thereby leading to increased activity in PVT Glu →BLA Glu neuronal circuit, which mediate obesity-related pain

Article Snippet: Seven days later, a volume of 50 ng/μl CXCL7 protein (HY- P75698 , MCE, USA) dissolved in artificial cerebrospinal fluid (ACSF) was infused into the lateral ventricles (1 μl), or PVT (300 nl) was given once a day for three days in a row.

Techniques: Expressing, Binding Assay, Activity Assay